Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2952194..2952784 Replicon chromosome
Accession NZ_CP119165
Organism Citrobacter freundii strain 2075

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag P0S03_RS14605 Protein ID WP_003836692.1
Coordinates 2952194..2952526 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag P0S03_RS14610 Protein ID WP_003836694.1
Coordinates 2952527..2952784 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P0S03_RS14580 (2947947) 2947947..2949434 + 1488 WP_003846749.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
P0S03_RS14590 (2949750) 2949750..2950700 - 951 WP_003846751.1 HTH-type transcriptional regulator Cbl -
P0S03_RS14595 (2950802) 2950802..2951719 - 918 WP_171859202.1 nitrogen assimilation transcriptional regulator NAC -
P0S03_RS14605 (2952194) 2952194..2952526 - 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
P0S03_RS14610 (2952527) 2952527..2952784 - 258 WP_003836694.1 hypothetical protein Antitoxin
P0S03_RS14615 (2953398) 2953398..2954483 + 1086 WP_238788026.1 hypothetical protein -
P0S03_RS14620 (2954594) 2954594..2957296 + 2703 WP_283903329.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2941797..2975059 33262


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T273877 WP_003836692.1 NZ_CP119165:c2952526-2952194 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT273877 WP_003836694.1 NZ_CP119165:c2952784-2952527 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References