Detailed information of TA system TA027387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 65748..66307
• Replicon: plasmid pRK1
• Accession: NC_017637
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E0J3F0
• Locus tag: ECW_RS24850
• Protein ID: WP_000421267.1
• Coordinates: 66032..66307 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: ECW_RS24845
• Protein ID: WP_001178087.1
• Coordinates: 65748..66032 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECW_RS24820	61398..61931	-	534	WP_001350011.1	transcription termination factor NusG	-
ECW_RS24825	62373..62660	-	288	WP_000074853.1	hypothetical protein	-
ECW_RS25205	62578..62841	-	264	Protein_65	ash family protein	-
ECW_RS24840	63813..64844	+	1032	WP_000907868.1	replication initiation protein	-
ECW_RS24845	65748..66032	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
ECW_RS24850	66032..66307	+	276	WP_000421267.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECW_RS24855	66413..66700	+	288	WP_001105072.1	hypothetical protein	-
ECW_RS28070	66891..67157	-	267	WP_000627834.1	hypothetical protein	-
ECW_RS24865	67157..67651	-	495	WP_001350009.1	hypothetical protein	-
ECW_RS24870	67707..68309	-	603	WP_000517697.1	hypothetical protein	-
ECW_RS24875	68663..70009	+	1347	WP_001132027.1	hypothetical protein	-
ECW_RS24880	70287..70973	+	687	Protein_74	colicin-Ia domain protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeC / faeD / faeE / faeF / faeG	1..102536	102536
	-	flank	IS/Tn	-	-	71162..72349	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10788.48 Da
• Isoelectric Point: 9.9325

>T27387 WP_000421267.1 NC_017637:66032-66307 [Escherichia coli W]
MELKWTSKALSDLARLYDFLVLTSKPAAARTVQSLTHAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T27387 NC_017637:66032-66307 [Escherichia coli W]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGACCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACATGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT27387 WP_001178087.1 NC_017637:65748-66032 [Escherichia coli W]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT27387 NC_017637:65748-66032 [Escherichia coli W]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: U9Y625