27386

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 4775794..4777129 Replicon chromosome
Accession NC_017635
Organism Escherichia coli W

Toxin (Protein)


Gene name sanaT Uniprot ID E0J2C5
Locus tag ECW_RS23795 Protein ID WP_000342500.1
Coordinates 4776182..4777129 (+) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID E0J2C6
Locus tag ECW_RS23790 Protein ID WP_000152741.1
Coordinates 4775794..4776189 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECW_RS23760 4771559..4772512 + 954 WP_000290405.1 hypothetical protein -
ECW_RS23765 4772748..4773566 + 819 WP_000761991.1 DUF945 domain-containing protein -
ECW_RS23770 4773596..4774069 + 474 WP_001292742.1 DNA repair protein RadC -
ECW_RS23775 4774069..4774404 + 336 WP_000939438.1 type IV toxin-antitoxin system YeeU family antitoxin -
ECW_RS23780 4774435..4774776 + 342 WP_000691790.1 TA system toxin CbtA family protein -
ECW_RS23785 4774891..4775724 + 834 WP_001192746.1 DUF4942 domain-containing protein -
ECW_RS23790 4775794..4776189 + 396 WP_000152741.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
ECW_RS23795 4776182..4777129 + 948 WP_000342500.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
ECW_RS23800 4777522..4778787 + 1266 WP_001218329.1 integrase arm-type DNA-binding domain-containing protein -
ECW_RS23805 4778988..4780457 - 1470 WP_001022619.1 serine/threonine protein kinase -
ECW_RS23810 4780454..4780867 - 414 WP_001368082.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE / fimA / fimI / fimC 4758477..4807470 48993


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-303)

Antitoxin

Protein of unknown function DUF6088

(7-108)


Sequences

Toxin        


Download         Length: 316 a.a.        Molecular weight: 35562.71 Da        Isoelectric Point: 4.9737

>T27386 WP_000342500.1 NC_017635:4776182-4777129 [Escherichia coli W]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGSTGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Download         Length: 948 bp

>T27386 NC_017635:4776182-4777129 [Escherichia coli W]
ATGGATGATTTAACCACTCTTTTTTCTGAAGTGGCTGACGCGCTTGGTATTGAGTCTGTCGCAATTGTGGAAAAAGATCA
CTATATCGTTGAGCTTTTGCAATTGCTTAAACCTCTGGTGTTCGATACTCATCAATTGGTGTTTGCCGGAGGAACCGCTC
TGGCAAAATCAGGTGTCGCACTTAATCGCATGTCGGAAGATGTGGATATCAAACTGGTGCCTAAGGATGCATTTCAGGCA
CTTTCCCGCACTCAGCGAAAAAATACCCGTAAAGAAATTGTTCAGGCAGTTTCCAGTGCAATAGCTTCATCGGGTGTGTT
CAATTTTGATGATGATTACCCAAAAGTGACGCGGGATGAATATCGTTATAATGACATACCTGTTCGTTATCCACAGCGAG
TGGTGCAGGCACCTTGTTTGCGTCCTTTTATCCGGCTGGAACTGATGGAAGCGAAGTTACTGGAGCCTTCTGAATTACGG
GATATTCATTCACTGGTAACAGGGAGCACGGGAAAAGCGGCTATTGTTTCAGCGTTTCCATGCGCAACTATCATTAGCAC
ACAGGCTGAAAAGCTGGTGGCGATGTTACGTCGTACTGCGGCAGTTGTGCGAGATGTTGAGCGATTTGATGATGCATCGC
TGGTACGTCATATCTACGATACAAACTGTATTGTTGAGACGAGTCATGTTGATATGAGTCTGCTCGTTGATTTTGTTCAA
CAAACTATTGAGCAGGACATTCAGCGTTACGGCAACCAGTACCCGCAGTTTTGTGCGTCTCCAGTTGATGAGCTAAAAAT
GGGACTGGATGAGTTGGCGAACAATCCTCTCTACCAGATGCGCTATCAGCAGTTTGTTTCGCCAATGGTGTATGGAGAGC
GAAAGATCACATGGGATGAGGCTTACTCCAGGTTTAGGAGCTTAGCGCTAGCCATCCTGAATGCTTGA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14619.93 Da        Isoelectric Point: 11.6915

>AT27386 WP_000152741.1 NC_017635:4775794-4776189 [Escherichia coli W]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRELVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Download         Length: 396 bp

>AT27386 NC_017635:4775794-4776189 [Escherichia coli W]
ATGACGATAAAAGCACGCATTCAGTCACGACTGAAACGTTCTAAACGTTACGTATTCACTCGTGACGACTTTAAAGATAT
TGCAGGCTACGATCAGGTTGGTCGGGTATTGCGCGAGCTTGTCAGGGAAGGGCAACTGTTGAAGGTTGGTTATGGCGTGT
ATACCAAAGCACGGCGGAACGGTATTACAGGCAAGGTAATGCCAGCGGCTCCCGGAGGCTCTTCAGCGGTGATTGTCGAA
ACGCTGGAACGCCTGAAAGTACGCTATCGTTTTGCTGGCGCAACGCAGGCATACAATAGCGGTACTTCTACGCAGATCCC
AGCTTCACCCGAGATAAAAACCCCCCGGCGTTTTAAACGCGTATTATCTGTAGGGAACAGCAGATTAAATGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E0J2C5

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4ZXD4

References