Detailed information of TA system TA027385

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4774069..4774776
• Replicon: chromosome
• Accession: NC_017635
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: E0J2C8
• Locus tag: ECW_RS23780
• Protein ID: WP_000691790.1
• Coordinates: 4774435..4774776 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ECW_RS23775
• Protein ID: WP_000939438.1
• Coordinates: 4774069..4774404 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECW_RS23750	4770016..4770234	-	219	WP_001064742.1	AlpA family phage regulatory protein	-
ECW_RS23755	4770352..4770966	-	615	WP_000772909.1	inovirus Gp2 family protein	-
ECW_RS23760	4771559..4772512	+	954	WP_000290405.1	hypothetical protein	-
ECW_RS23765	4772748..4773566	+	819	WP_000761991.1	DUF945 domain-containing protein	-
ECW_RS23770	4773596..4774069	+	474	WP_001292742.1	DNA repair protein RadC	-
ECW_RS23775	4774069..4774404	+	336	WP_000939438.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECW_RS23780	4774435..4774776	+	342	WP_000691790.1	TA system toxin CbtA family protein	Toxin
ECW_RS23785	4774891..4775724	+	834	WP_001192746.1	DUF4942 domain-containing protein	-
ECW_RS23790	4775794..4776189	+	396	WP_000152741.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
ECW_RS23795	4776182..4777129	+	948	WP_000342500.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
ECW_RS23800	4777522..4778787	+	1266	WP_001218329.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	4758477..4807470	48993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13060.08 Da
• Isoelectric Point: 9.4583

>T27385 WP_000691790.1 NC_017635:4774435-4774776 [Escherichia coli W]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCYNTTAR

Nucleotide

• Length: 342 bp

>T27385 NC_017635:4774435-4774776 [Escherichia coli W]
ATGAAAATTATACCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCTG
CTATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12639.63 Da
• Isoelectric Point: 7.7723

>AT27385 WP_000939438.1 NC_017635:4774069-4774404 [Escherichia coli W]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPWQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT27385 NC_017635:4774069-4774404 [Escherichia coli W]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGGCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: E0J2C8