Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3799152..3799952 Replicon chromosome
Accession NC_017635
Organism Escherichia coli W

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag ECW_RS19035 Protein ID WP_000342449.1
Coordinates 3799152..3799679 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag ECW_RS19040 Protein ID WP_001277108.1
Coordinates 3799686..3799952 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECW_RS19010 3794227..3794994 - 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
ECW_RS19015 3794991..3796268 - 1278 WP_000803798.1 branched chain amino acid ABC transporter permease LivM -
ECW_RS19020 3796265..3797191 - 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
ECW_RS19025 3797239..3798348 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
ECW_RS19030 3798772..3799155 + 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
ECW_RS19035 3799152..3799679 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
ECW_RS19040 3799686..3799952 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
ECW_RS19045 3800102..3801205 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
ECW_RS19050 3801476..3802330 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
ECW_RS19055 3802575..3803633 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
ECW_RS19060 3803626..3804294 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T27380 WP_000342449.1 NC_017635:c3799679-3799152 [Escherichia coli W]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T27380 NC_017635:c3799679-3799152 [Escherichia coli W]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT27380 WP_001277108.1 NC_017635:c3799952-3799686 [Escherichia coli W]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT27380 NC_017635:c3799952-3799686 [Escherichia coli W]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References