Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 442981..443478 Replicon chromosome
Accession NZ_CP119120
Organism Escherichia coli strain GENOMIC22-004

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PYZ88_RS02065 Protein ID WP_001173343.1
Coordinates 443305..443478 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag PYZ88_RS02060 Protein ID WP_001425728.1
Coordinates 442981..443277 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PYZ88_RS02040 (439712) 439712..440431 - 720 WP_000611288.1 two-component system response regulator DcuR -
PYZ88_RS02045 (440428) 440428..442059 - 1632 WP_001216487.1 sensor histidine kinase -
PYZ88_RS02050 (442240) 442240..442470 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
PYZ88_RS02055 (442482) 442482..442754 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
PYZ88_RS02060 (442981) 442981..443277 + 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PYZ88_RS02065 (443305) 443305..443478 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PYZ88_RS02070 (443597) 443597..445114 - 1518 WP_001295074.1 lysine--tRNA ligase -
PYZ88_RS02075 (445351) 445351..446808 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T273780 WP_001173343.1 NZ_CP119120:443305-443478 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T273780 NZ_CP119120:443305-443478 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT273780 WP_001425728.1 NZ_CP119120:442981-443277 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT273780 NZ_CP119120:442981-443277 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References