Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2767805..2768430 Replicon chromosome
Accession NC_017635
Organism Escherichia coli W

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag ECW_RS13880 Protein ID WP_000911329.1
Coordinates 2767805..2768203 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag ECW_RS13885 Protein ID WP_000450524.1
Coordinates 2768203..2768430 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECW_RS13860 2763712..2763912 + 201 WP_000383836.1 YpfN family protein -
ECW_RS13865 2763993..2764691 - 699 WP_000679823.1 esterase -
ECW_RS13870 2764765..2766780 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
ECW_RS13875 2766795..2767658 - 864 WP_001267498.1 neutral zinc metallopeptidase -
ECW_RS13880 2767805..2768203 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
ECW_RS13885 2768203..2768430 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
ECW_RS13890 2768585..2769298 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
ECW_RS13900 2769511..2770545 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
ECW_RS13905 2770562..2771440 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
ECW_RS13910 2771586..2772158 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
ECW_RS13915 2772158..2772628 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T27376 WP_000911329.1 NC_017635:c2768203-2767805 [Escherichia coli W]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T27376 NC_017635:c2768203-2767805 [Escherichia coli W]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT27376 WP_000450524.1 NC_017635:c2768430-2768203 [Escherichia coli W]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT27376 NC_017635:c2768430-2768203 [Escherichia coli W]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References