Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5418438..5419063 Replicon chromosome
Accession NZ_CP119013
Organism Klebsiella pneumoniae strain CR-hvKP005

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag PY729_RS26800 Protein ID WP_002882817.1
Coordinates 5418438..5418821 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag PY729_RS26805 Protein ID WP_004150355.1
Coordinates 5418821..5419063 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PY729_RS26785 (PY729_26785) 5415804..5416706 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
PY729_RS26790 (PY729_26790) 5416703..5417338 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
PY729_RS26795 (PY729_26795) 5417335..5418264 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
PY729_RS26800 (PY729_26800) 5418438..5418821 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
PY729_RS26805 (PY729_26805) 5418821..5419063 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
PY729_RS26810 (PY729_26810) 5419268..5420185 + 918 WP_002882812.1 alpha/beta hydrolase -
PY729_RS26815 (PY729_26815) 5420199..5421140 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
PY729_RS26820 (PY729_26820) 5421185..5421622 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
PY729_RS26825 (PY729_26825) 5421619..5422479 - 861 WP_002882807.1 virulence factor BrkB family protein -
PY729_RS26830 (PY729_26830) 5422473..5423072 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T273719 WP_002882817.1 NZ_CP119013:c5418821-5418438 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT273719 WP_004150355.1 NZ_CP119013:c5419063-5418821 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References