Detailed information of TA system TA273697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 514429..516143
• Replicon: chromosome
• Accession: NZ_CP118988
• Organism: Aeromonas allosaccharophila strain K520

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: PYU98_RS02535
• Protein ID: WP_182938866.1
• Coordinates: 514429..515082 (+)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: A0A8I0BGM7
• Locus tag: PYU98_RS02540
• Protein ID: WP_025201144.1
• Coordinates: 515079..516143 (+)
• Length: 355 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PYU98_RS02515 (PYU98_02515)	509460..509648	+	189	WP_021138010.1	DUF3283 family protein	-
PYU98_RS02520 (PYU98_02520)	509795..510241	+	447	WP_043852095.1	hypothetical protein	-
PYU98_RS02525 (PYU98_02525)	510428..512875	+	2448	WP_113995997.1	type I restriction-modification system subunit M	-
PYU98_RS02530 (PYU98_02530)	512875..514152	+	1278	WP_113995996.1	restriction endonuclease subunit S	-
PYU98_RS02535 (PYU98_02535)	514429..515082	+	654	WP_182938866.1	DUF4433 domain-containing protein	Toxin
PYU98_RS02540 (PYU98_02540)	515079..516143	+	1065	WP_025201144.1	macro domain-containing protein	Antitoxin
PYU98_RS02545 (PYU98_02545)	516218..519349	+	3132	WP_275057355.1	HsdR family type I site-specific deoxyribonuclease	-
PYU98_RS02550 (PYU98_02550)	519349..520062	+	714	WP_005331303.1	SprT family zinc-dependent metalloprotease	-
PYU98_RS02555 (PYU98_02555)	520081..520329	-	249	WP_232093607.1	restriction endonuclease	-
PYU98_RS02560 (PYU98_02560)	520212..521018	-	807	WP_232093608.1	winged helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	494096..525077	30981

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-216)

Antitoxin

• Macro domain (18-131)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 25484.13 Da
• Isoelectric Point: 7.0177

>T273697 WP_182938866.1 NZ_CP118988:514429-515082 [Aeromonas allosaccharophila]
MAYDYSQSLNPQKALIFRIVHRDNLEWILTHGLWAGNGQQQNPHWVHIGLPELIAKRARHPVPLVPYGVLNDYVPFYFTP
FSPMLSNIHSGRGVPRRENDEILILVSSLHRMPELGLPFVFTDSHAYYQWANFYAELGDLERIDWPLLQRRDFKRDADDL
SKLERYQAEALIHQHCPIEALLGVVCYNEEIKNRVEQCMNRVGVARPVYVRDGWYFR

Nucleotide

• Length: 654 bp

Antitoxin

Protein

• Length: 355 a.a.
• Molecular weight: 39563.71 Da
• Isoelectric Point: 6.1433

>AT273697 WP_025201144.1 NZ_CP118988:515079-516143 [Aeromonas allosaccharophila]
MIRYTTGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPANMRAYAEACKQGDVVTGKMFITQTGELLGPRWIVNFPTK
QHWRNPAQMAWIQDGLQDLRAWLQAQQVSSIAIPPLGAGNGGLPWPEVKQAIETALGDLDIDILLYEPSSQYQNVAKRGG
VAELTPARALIAELVRRYWFLGMECSLLEIQKLAWFLARVIGLNPLGDPLQLQFKAHRYGPYANNLTHLLNAMDGSYLQS
DKRIPDSKPFDVIWFNEAKKQELAVYLNTEAKAFLPILEQTTALIDGFESPYGMELLATVDWLMVEEGCAPTLSALREGL
HRWPAGTPWGDRKARLFDDRSLQIALTRLQQGLV

Nucleotide

• Length: 1065 bp