Detailed information of TA system TA027366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1785341..1785867
• Replicon: chromosome
• Accession: NC_017635
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: ECW_RS08840
• Protein ID: WP_000323025.1
• Coordinates: 1785341..1785628 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: ECW_RS08845
• Protein ID: WP_000534858.1
• Coordinates: 1785628..1785867 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECW_RS08800	1780365..1780580	-	216	WP_000839590.1	phage lysis protein EssD	-
ECW_RS08805	1781334..1781549	-	216	WP_000066484.1	cold shock-like protein CspB	-
ECW_RS08810	1781850..1782062	+	213	WP_000087756.1	cold shock-like protein CspF	-
ECW_RS27565	1782117..1782206	+	90	WP_120795389.1	hypothetical protein	-
ECW_RS08815	1782484..1783236	-	753	WP_001047135.1	antitermination protein	-
ECW_RS08820	1783250..1784299	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
ECW_RS08825	1784301..1784579	-	279	WP_012304870.1	hypothetical protein	-
ECW_RS08830	1784646..1784897	-	252	WP_000980994.1	hypothetical protein	-
ECW_RS08835	1785114..1785269	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
ECW_RS08840	1785341..1785628	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
ECW_RS08845	1785628..1785867	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
ECW_RS25870	1785892..1786197	+	306	WP_001326990.1	hypothetical protein	-
ECW_RS08850	1786400..1786732	+	333	WP_001301033.1	protein FlxA	-
ECW_RS08855	1787169..1788482	-	1314	WP_000589005.1	ISNCY family transposase	-
ECW_RS27960	1789251..1789538	-	288	Protein_1690	hypothetical protein	-
ECW_RS08870	1790149..1790505	-	357	WP_001310834.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1749941..1798990	49049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T27366 WP_000323025.1 NC_017635:c1785628-1785341 [Escherichia coli W]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T27366 NC_017635:c1785628-1785341 [Escherichia coli W]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT27366 WP_000534858.1 NC_017635:c1785867-1785628 [Escherichia coli W]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT27366 NC_017635:c1785867-1785628 [Escherichia coli W]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8