Detailed information of TA system TA027364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1615101..1615739
• Replicon: chromosome
• Accession: NC_017635
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECW_RS08020
• Protein ID: WP_000813794.1
• Coordinates: 1615101..1615277 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECW_RS08025
• Protein ID: WP_001270285.1
• Coordinates: 1615323..1615739 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECW_RS08000	1610720..1611934	-	1215	WP_001326689.1	BenE family transporter YdcO	-
ECW_RS08005	1611987..1612523	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECW_RS08010	1612596..1614557	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECW_RS08015	1614649..1614879	-	231	WP_000494244.1	YncJ family protein	-
ECW_RS08020	1615101..1615277	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECW_RS08025	1615323..1615739	+	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECW_RS08030	1615818..1617224	+	1407	WP_000760589.1	PLP-dependent aminotransferase family protein	-
ECW_RS08035	1617469..1618614	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
ECW_RS08040	1618632..1619645	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
ECW_RS08045	1619646..1620587	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1609532..1610680	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T27364 WP_000813794.1 NC_017635:1615101-1615277 [Escherichia coli W]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T27364 NC_017635:1615101-1615277 [Escherichia coli W]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT27364 WP_001270285.1 NC_017635:1615323-1615739 [Escherichia coli W]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT27364 NC_017635:1615323-1615739 [Escherichia coli W]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB