Detailed information of TA system TA273638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sezT-pezT/zeta-couple_hipB
• Location: 1406024..1407288
• Replicon: chromosome
• Accession: NZ_CP118957
• Organism: Enterococcus saccharolyticus subsp. saccharolyticus strain DSM 20726

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: S0NXW2
• Locus tag: PYW32_RS07325
• Protein ID: WP_016174967.1
• Coordinates: 1406024..1406812 (-)
• Length: 263 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: PYW32_RS07330
• Protein ID: WP_016174966.1
• Coordinates: 1406812..1407288 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PYW32_RS07295	1401720..1402007	+	288	WP_016174973.1	hypothetical protein	-
PYW32_RS07300	1402178..1402411	-	234	WP_016174972.1	heavy-metal-associated domain-containing protein	-
PYW32_RS07305	1402485..1404332	-	1848	WP_016174971.1	heavy metal translocating P-type ATPase	-
PYW32_RS07310	1404384..1404686	-	303	WP_016174970.1	hypothetical protein	-
PYW32_RS07315	1404851..1405600	-	750	WP_016174969.1	Crp/Fnr family transcriptional regulator	-
PYW32_RS07320	1405822..1406034	-	213	WP_016174968.1	AAA family ATPase	-
PYW32_RS07325	1406024..1406812	-	789	WP_016174967.1	type II toxin-antitoxin system toxin PezT	Toxin
PYW32_RS07330	1406812..1407288	-	477	WP_016174966.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
PYW32_RS07335	1407358..1407591	-	234	Protein_1401	hypothetical protein	-
PYW32_RS07340	1407585..1407892	-	308	Protein_1402	transposase	-
PYW32_RS07345	1407962..1408924	-	963	WP_016174964.1	hypothetical protein	-
PYW32_RS07350	1408938..1409159	-	222	WP_016174963.1	YdbC family protein	-
PYW32_RS07355	1409177..1409521	-	345	WP_016174962.1	nucleotide pyrophosphohydrolase	-
PYW32_RS07360	1409563..1410771	-	1209	WP_016174961.1	DUF3578 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1403382..1487304	83922
	-	flank	IS/Tn	-	-	1407599..1407892	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 263 a.a.
• Molecular weight: 29830.73 Da
• Isoelectric Point: 5.4749

>T273638 WP_016174967.1 NZ_CP118957:c1406812-1406024 [Enterococcus saccharolyticus subsp. saccharolyticus]
MRLEEFSEEEFQKALQRTIRALTRGKTIPGQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEQLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLASKGYQVSLAVIGTKSELSYLSTLIRYEE
LYAIDPNQARATPKEHHDGIVENLVDNLRELESEKLFEQIQIYQRDRACIYDSETDKDSAAEVLQDCLFGEWSKVEEEML
KLGRERLVKLNNKNLLESNYGI

Nucleotide

• Length: 789 bp

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18252.66 Da
• Isoelectric Point: 4.3965

>AT273638 WP_016174966.1 NZ_CP118957:c1407288-1406812 [Enterococcus saccharolyticus subsp. saccharolyticus]
MIGDNIKSLRHTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYIDIVGEDKMLTPVEDYQLTLKVE
VIKERGAAILSQLYRYQDSQDIAFDDEANPWILMSDDLAELINTKIYLVDTFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

Structures

Toxin

• AlphaFold DB: S0NXW2