Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 44561..45297 Replicon plasmid pRX.G5M15_1
Accession NZ_CP118937
Organism Klebsiella pneumoniae subsp. pneumoniae strain RX.G5M15

Toxin (Protein)


Gene name tacT Uniprot ID A0A443XD13
Locus tag PX704_RS25515 Protein ID WP_075253142.1
Coordinates 44815..45297 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag PX704_RS25510 Protein ID WP_003026799.1
Coordinates 44561..44827 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PX704_RS25500 (PX704_25505) 40494..42125 - 1632 WP_087846553.1 carbohydrate porin -
PX704_RS25505 (PX704_25510) 42545..43513 + 969 WP_275107659.1 IS5 family transposase -
PX704_RS25510 (PX704_25515) 44561..44827 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
PX704_RS25515 (PX704_25520) 44815..45297 + 483 WP_075253142.1 GNAT family N-acetyltransferase Toxin
PX704_RS25520 (PX704_25525) 45469..47001 + 1533 WP_275107661.1 IS3-like element ISKpn38 family transposase -
PX704_RS25525 (PX704_25530) 47027..47383 - 357 Protein_47 transposase -
PX704_RS25530 (PX704_25535) 48004..49173 + 1170 WP_048977890.1 efflux RND transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..152213 152213
- inside IScluster/Tn - - 42545..47383 4838


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17254.90 Da        Isoelectric Point: 8.7400

>T273618 WP_075253142.1 NZ_CP118937:44815-45297 [Klebsiella pneumoniae subsp. pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT273618 WP_003026799.1 NZ_CP118937:44561-44827 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A443XD13

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References