Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5136093..5136718 Replicon chromosome
Accession NZ_CP118936
Organism Klebsiella pneumoniae subsp. pneumoniae strain RX.G5M15

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag PX704_RS24860 Protein ID WP_002882817.1
Coordinates 5136093..5136476 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag PX704_RS24865 Protein ID WP_004150355.1
Coordinates 5136476..5136718 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PX704_RS24845 (5133459) 5133459..5134361 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
PX704_RS24850 (5134358) 5134358..5134993 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
PX704_RS24855 (5134990) 5134990..5135919 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
PX704_RS24860 (5136093) 5136093..5136476 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
PX704_RS24865 (5136476) 5136476..5136718 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
PX704_RS24870 (5136923) 5136923..5137840 + 918 WP_032422045.1 alpha/beta hydrolase -
PX704_RS24875 (5137854) 5137854..5138795 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
PX704_RS24880 (5138840) 5138840..5139277 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
PX704_RS24885 (5139274) 5139274..5140134 - 861 WP_004146232.1 virulence factor BrkB family protein -
PX704_RS24890 (5140128) 5140128..5140727 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T273617 WP_002882817.1 NZ_CP118936:c5136476-5136093 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT273617 WP_004150355.1 NZ_CP118936:c5136718-5136476 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References