Detailed information of TA system TA273580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4621130..4621746
• Replicon: chromosome
• Accession: NZ_CP118927
• Organism: Citrobacter koseri strain L2395

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A8AL49
• Locus tag: PX343_RS21895
• Protein ID: WP_012133919.1
• Coordinates: 4621130..4621504 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: PX343_RS21900
• Protein ID: WP_162790489.1
• Coordinates: 4621501..4621746 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PX343_RS21880 (PX343_21880)	4618633..4619535	+	903	WP_071259455.1	formate dehydrogenase O subunit beta	-
PX343_RS21885 (PX343_21885)	4619532..4620167	+	636	WP_049011887.1	formate dehydrogenase cytochrome b556 subunit	-
PX343_RS21890 (PX343_21890)	4620164..4621093	+	930	WP_047459875.1	formate dehydrogenase accessory protein FdhE	-
PX343_RS21895 (PX343_21895)	4621130..4621504	-	375	WP_012133919.1	type II toxin-antitoxin system VapC family toxin	Toxin
PX343_RS21900 (PX343_21900)	4621501..4621746	-	246	WP_162790489.1	CopG family transcriptional regulator	Antitoxin
PX343_RS21905 (PX343_21905)	4621982..4622923	-	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
PX343_RS21910 (PX343_21910)	4622968..4623405	-	438	WP_047459887.1	D-aminoacyl-tRNA deacylase	-
PX343_RS21915 (PX343_21915)	4623402..4624274	-	873	WP_012133924.1	virulence factor BrkB family protein	-
PX343_RS21920 (PX343_21920)	4624268..4624867	-	600	WP_047459890.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13741.00 Da
• Isoelectric Point: 9.8061

>T273580 WP_012133919.1 NZ_CP118927:c4621504-4621130 [Citrobacter koseri]
MIKGPVLFDTNILIDLFSGRGEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T273580 NZ_CP118927:c4621504-4621130 [Citrobacter koseri]
ATGATTAAAGGTCCGGTGTTGTTTGACACCAATATTCTCATTGATTTATTTAGCGGGCGTGGTGAAGCGAAGAAGGCGCT
CGAAGCTTACTCACCCCAGAATGCCATTAGTCTCATTACCTGGATGGAAGTGATGGTAGGGGCAAAAAAGTACCATCAGG
AATCCCGCACACGTATAGCATTGAGCGCTTTTAACGTTATTGGCGTTTCACAGGATATTGCCGAAAGAAGTGTCAGTCTC
AGGCAGGAATACGGGATGAAGTTGCCGGATGCGGTTATTCTGGCTACAGCGCACATTCATCGTTTTGCGCTCGTGACGCG
CAATACAAAGGACTTTGCGGGGATTCCGGGAGTCATTACGCCTTACCATTTATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9524.75 Da
• Isoelectric Point: 4.1961

>AT273580 WP_162790489.1 NZ_CP118927:c4621746-4621501 [Citrobacter koseri]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW
Q

Nucleotide

• Length: 246 bp

>AT273580 NZ_CP118927:c4621746-4621501 [Citrobacter koseri]
ATGATGGCTGGAATGGAAATGGGCAGAATATTACTTGATTTATCAGATGAGGTTATCAAGCGGCTTGATGACCTCAAACA
ACAGCGTAATCTTCCGCGAGCAGAGCTATTACGCGAAGCAGTTGAACAATACCTGGAAAGGCAGGGACAAGCCGAAACCA
CTATTTCCGATGCGCTGGGGCTCTGGCAAGGTTGCGAAGAAGATGGCGTTGAATACGAAAGGAAGCTCCGCGAGGAGTGG
CAATGA

Structures

Toxin

• AlphaFold DB: A8AL49