Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2435529..2436166 Replicon chromosome
Accession NZ_CP118911
Organism Bacillus velezensis strain XRD006

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PXG99_RS12205 Protein ID WP_003156187.1
Coordinates 2435529..2435879 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag PXG99_RS12210 Protein ID WP_003156188.1
Coordinates 2435885..2436166 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PXG99_RS12165 (2430569) 2430569..2431171 - 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -
PXG99_RS12170 (2431171) 2431171..2431959 - 789 WP_029974100.1 RNA polymerase sigma factor SigB -
PXG99_RS12175 (2431925) 2431925..2432407 - 483 WP_007609591.1 anti-sigma B factor RsbW -
PXG99_RS12180 (2432404) 2432404..2432733 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
PXG99_RS12185 (2432797) 2432797..2433804 - 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
PXG99_RS12190 (2433816) 2433816..2434217 - 402 WP_003156178.1 anti-sigma regulatory factor -
PXG99_RS12195 (2434220) 2434220..2434585 - 366 WP_007609584.1 RsbT antagonist protein RsbS -
PXG99_RS12200 (2434590) 2434590..2435411 - 822 WP_003156182.1 STAS domain-containing protein -
PXG99_RS12205 (2435529) 2435529..2435879 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PXG99_RS12210 (2435885) 2435885..2436166 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PXG99_RS12215 (2436286) 2436286..2437455 - 1170 WP_021495079.1 alanine racemase -
PXG99_RS12220 (2437572) 2437572..2438579 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
PXG99_RS12225 (2438744) 2438744..2439109 - 366 WP_007609580.1 holo-ACP synthase -
PXG99_RS12230 (2439202) 2439202..2439801 + 600 WP_029974099.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T273566 WP_003156187.1 NZ_CP118911:c2435879-2435529 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT273566 WP_003156188.1 NZ_CP118911:c2436166-2435885 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References