Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5267042..5267667 Replicon chromosome
Accession NZ_CP118901
Organism Klebsiella pneumoniae strain CRKP

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag PXG74_RS25560 Protein ID WP_002882817.1
Coordinates 5267042..5267425 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag PXG74_RS25565 Protein ID WP_004150355.1
Coordinates 5267425..5267667 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PXG74_RS25545 (5264408) 5264408..5265310 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
PXG74_RS25550 (5265307) 5265307..5265942 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
PXG74_RS25555 (5265939) 5265939..5266868 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
PXG74_RS25560 (5267042) 5267042..5267425 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
PXG74_RS25565 (5267425) 5267425..5267667 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
PXG74_RS25570 (5267872) 5267872..5268789 + 918 WP_032422045.1 alpha/beta hydrolase -
PXG74_RS25575 (5268803) 5268803..5269744 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
PXG74_RS25580 (5269789) 5269789..5270226 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
PXG74_RS25585 (5270223) 5270223..5271083 - 861 WP_004146232.1 virulence factor BrkB family protein -
PXG74_RS25590 (5271077) 5271077..5271676 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T273543 WP_002882817.1 NZ_CP118901:c5267425-5267042 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT273543 WP_004150355.1 NZ_CP118901:c5267667-5267425 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References