Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4667162..4667972 Replicon chromosome
Accession NZ_CP118901
Organism Klebsiella pneumoniae strain CRKP

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag PXG74_RS22825 Protein ID WP_004178461.1
Coordinates 4667162..4667695 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag PXG74_RS22830 Protein ID WP_002887278.1
Coordinates 4667706..4667972 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PXG74_RS22820 (4665993) 4665993..4667114 + 1122 WP_004186850.1 cupin domain-containing protein -
PXG74_RS22825 (4667162) 4667162..4667695 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
PXG74_RS22830 (4667706) 4667706..4667972 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
PXG74_RS22835 (4668075) 4668075..4669508 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
PXG74_RS22840 (4669498) 4669498..4670181 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
PXG74_RS22845 (4670354) 4670354..4671739 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
PXG74_RS22850 (4671757) 4671757..4672101 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T273540 WP_004178461.1 NZ_CP118901:c4667695-4667162 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT273540 WP_002887278.1 NZ_CP118901:c4667972-4667706 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References