Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4419811..4420308 Replicon chromosome
Accession NC_017634
Organism Escherichia coli O83:H1 str. NRG 857C

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NRG857_RS21190 Protein ID WP_001173343.1
Coordinates 4420135..4420308 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag NRG857_RS21185 Protein ID WP_001312331.1
Coordinates 4419811..4420107 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRG857_RS21165 4416540..4417259 - 720 WP_000611291.1 two-component system response regulator DcuR -
NRG857_RS21170 4417256..4418887 - 1632 WP_001216466.1 sensor histidine kinase -
NRG857_RS21175 4419068..4419298 + 231 WP_000373294.1 (4Fe-4S)-binding protein -
NRG857_RS21180 4419310..4419582 + 273 WP_000405647.1 N-acetyltransferase -
NRG857_RS21185 4419811..4420107 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NRG857_RS21190 4420135..4420308 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NRG857_RS21195 4420427..4421944 - 1518 WP_000003805.1 lysine--tRNA ligase -
NRG857_RS21200 4422181..4423638 - 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T27352 WP_001173343.1 NC_017634:4420135-4420308 [Escherichia coli O83:H1 str. NRG 857C]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T27352 NC_017634:4420135-4420308 [Escherichia coli O83:H1 str. NRG 857C]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT27352 WP_001312331.1 NC_017634:4419811-4420107 [Escherichia coli O83:H1 str. NRG 857C]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT27352 NC_017634:4419811-4420107 [Escherichia coli O83:H1 str. NRG 857C]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References