Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3015644..3016298 Replicon chromosome
Accession NC_017634
Organism Escherichia coli O83:H1 str. NRG 857C

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NRG857_RS14535 Protein ID WP_000244781.1
Coordinates 3015644..3016051 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NRG857_RS14540 Protein ID WP_000354046.1
Coordinates 3016032..3016298 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRG857_RS14515 3011601..3013334 - 1734 WP_000813193.1 single-stranded-DNA-specific exonuclease RecJ -
NRG857_RS14520 3013340..3014050 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NRG857_RS14525 3014075..3014971 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NRG857_RS14530 3015083..3015604 + 522 WP_001055874.1 flavodoxin FldB -
NRG857_RS14535 3015644..3016051 - 408 WP_000244781.1 protein YgfX Toxin
NRG857_RS14540 3016032..3016298 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NRG857_RS14545 3016541..3017521 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
NRG857_RS14550 3017598..3018257 - 660 WP_000250274.1 hemolysin III family protein -
NRG857_RS14555 3018421..3018732 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
NRG857_RS14560 3018777..3020210 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T27347 WP_000244781.1 NC_017634:c3016051-3015644 [Escherichia coli O83:H1 str. NRG 857C]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T27347 NC_017634:c3016051-3015644 [Escherichia coli O83:H1 str. NRG 857C]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT27347 WP_000354046.1 NC_017634:c3016298-3016032 [Escherichia coli O83:H1 str. NRG 857C]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT27347 NC_017634:c3016298-3016032 [Escherichia coli O83:H1 str. NRG 857C]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATTCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References