Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2063636..2064226 Replicon chromosome
Accession NC_017634
Organism Escherichia coli O83:H1 str. NRG 857C

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag NRG857_RS10225 Protein ID WP_000373724.1
Coordinates 2063636..2063968 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag NRG857_RS10230 Protein ID WP_000288812.1
Coordinates 2063969..2064226 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRG857_RS10200 2059099..2060361 + 1263 Protein_1995 integrase arm-type DNA-binding domain-containing protein -
NRG857_RS10205 2060500..2060958 - 459 WP_000526101.1 IS200/IS605 family transposase -
NRG857_RS10210 2061910..2062788 - 879 WP_000511773.1 integrase domain-containing protein -
NRG857_RS10215 2062926..2063252 + 327 Protein_1998 hypothetical protein -
NRG857_RS10220 2063233..2063589 + 357 WP_001147230.1 hypothetical protein -
NRG857_RS10225 2063636..2063968 - 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRG857_RS10230 2063969..2064226 - 258 WP_000288812.1 antitoxin Antitoxin
NRG857_RS23595 2064324..2064515 + 192 WP_077561361.1 hypothetical protein -
NRG857_RS10240 2064574..2064780 - 207 WP_000983730.1 helix-turn-helix domain-containing protein -
NRG857_RS10245 2064777..2065244 - 468 WP_000201267.1 hypothetical protein -
NRG857_RS10250 2065477..2066613 - 1137 WP_000420971.1 ISAs1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2057618..2064780 7162
- inside IScluster/Tn - - 2060500..2066613 6113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T27344 WP_000373724.1 NC_017634:c2063968-2063636 [Escherichia coli O83:H1 str. NRG 857C]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T27344 NC_017634:c2063968-2063636 [Escherichia coli O83:H1 str. NRG 857C]
ATGGACAGAGGGGAAATCTGGCTGGTTTCACTGGATCCGATTGCAGGTCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCCGTTGTTGTTCCCGTTACCAGCGGCGGAAACTTCGCCCGTG
CAGCCGGTTTTACCGTCTCACTGGACGGTGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCCCGGAGCGGTAAGCGACTGGAACGTATACCCGATGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAGGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT27344 WP_000288812.1 NC_017634:c2064226-2063969 [Escherichia coli O83:H1 str. NRG 857C]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT27344 NC_017634:c2064226-2063969 [Escherichia coli O83:H1 str. NRG 857C]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCCCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGTTGTTGGCGCAGTGCGATCCGCACGCAGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References