273419

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 70472..71609 Replicon plasmid pW5-2
Accession NZ_CP118757
Organism Enterococcus faecalis strain W5

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag PWA41_RS14700 Protein ID WP_002332783.1
Coordinates 70472..71335 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag PWA41_RS14705 Protein ID WP_002326825.1
Coordinates 71337..71609 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWA41_RS14660 (PWA41_14655) 66318..66434 - 117 Protein_81 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
PWA41_RS14665 (PWA41_14660) 66604..67341 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
PWA41_RS14670 (PWA41_14665) 67466..67549 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
PWA41_RS14675 (PWA41_14670) 67672..67839 - 168 Protein_84 peptide-binding protein -
PWA41_RS14680 (PWA41_14675) 67973..68122 - 150 Protein_85 topoisomerase -
PWA41_RS14685 (PWA41_14680) 68198..68878 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
PWA41_RS14690 (PWA41_14685) 68912..69418 - 507 WP_002415429.1 trimethoprim-resistant dihydrofolate reductase DfrG -
PWA41_RS14695 (PWA41_14690) 69715..70032 - 318 WP_002326830.1 hypothetical protein -
PWA41_RS14700 (PWA41_14695) 70472..71335 - 864 WP_002332783.1 zeta toxin family protein Toxin
PWA41_RS14705 (PWA41_14700) 71337..71609 - 273 WP_002326825.1 antitoxin Antitoxin
PWA41_RS14710 (PWA41_14705) 71627..71842 - 216 WP_001835296.1 peptide-binding protein -
PWA41_RS14715 (PWA41_14710) 71934..72830 - 897 WP_002326827.1 ParA family protein -
PWA41_RS14720 (PWA41_14715) 72933..73193 - 261 Protein_93 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
PWA41_RS14725 (PWA41_14720) 73363..74100 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
PWA41_RS14730 (PWA41_14725) 74225..74308 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
PWA41_RS14735 (PWA41_14730) 74740..75111 - 372 WP_002358205.1 hypothetical protein -
PWA41_RS14740 (PWA41_14735) 75104..75949 - 846 WP_000239313.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(L) / tet(M) / fexA / optrA / erm(A) / erm(B) / aph(3')-III / ant(6)-Ia / dfrG - 1..76420 76420
- inside IS/Tn erm(B) / aph(3')-III / ant(6)-Ia / dfrG - 62478..74100 11622


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T273419 WP_002332783.1 NZ_CP118757:c71335-70472 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT273419 WP_002326825.1 NZ_CP118757:c71609-71337 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References