Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 579759..580395 Replicon chromosome
Accession NZ_CP118744
Organism Bacillus paralicheniformis strain RP01

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag PWG69_RS02905 Protein ID WP_003179128.1
Coordinates 580045..580395 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag PWG69_RS02900 Protein ID WP_006638778.1
Coordinates 579759..580040 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWG69_RS02880 (PWG69_02880) 574870..576354 + 1485 WP_023857868.1 PH domain-containing protein -
PWG69_RS02885 (PWG69_02885) 576351..576950 - 600 WP_023857867.1 rhomboid family intramembrane serine protease -
PWG69_RS02890 (PWG69_02890) 577294..578253 + 960 WP_164468292.1 outer membrane lipoprotein carrier protein LolA -
PWG69_RS02895 (PWG69_02895) 578478..579647 + 1170 WP_023857864.1 alanine racemase -
PWG69_RS02900 (PWG69_02900) 579759..580040 + 282 WP_006638778.1 hypothetical protein Antitoxin
PWG69_RS02905 (PWG69_02905) 580045..580395 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PWG69_RS02910 (PWG69_02910) 580513..581340 + 828 WP_025810067.1 RsbT co-antagonist protein RsbRA -
PWG69_RS02915 (PWG69_02915) 581344..581709 + 366 WP_020450259.1 STAS domain-containing protein -
PWG69_RS02920 (PWG69_02920) 581712..582113 + 402 WP_020450260.1 anti-sigma regulatory factor -
PWG69_RS02925 (PWG69_02925) 582124..583131 + 1008 WP_020450261.1 PP2C family protein-serine/threonine phosphatase -
PWG69_RS02930 (PWG69_02930) 583190..583516 + 327 WP_020450262.1 anti-sigma factor antagonist -
PWG69_RS02935 (PWG69_02935) 583516..583998 + 483 WP_020450263.1 anti-sigma B factor RsbW -
PWG69_RS02940 (PWG69_02940) 583964..584755 + 792 WP_023857858.1 RNA polymerase sigma factor SigB -
PWG69_RS02945 (PWG69_02945) 584752..585351 + 600 WP_020450265.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T273397 WP_003179128.1 NZ_CP118744:580045-580395 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT273397 WP_006638778.1 NZ_CP118744:579759-580040 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References