Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Xre(antitoxin)
Location 4526152..4527409 Replicon chromosome
Accession NZ_CP118638
Organism Pseudomonas aeruginosa strain P9

Toxin (Protein)


Gene name - Uniprot ID Q9I4U4
Locus tag PUN20_RS21030 Protein ID WP_003112528.1
Coordinates 4526152..4526907 (-) Length 252 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID V6AJU7
Locus tag PUN20_RS21035 Protein ID WP_003086329.1
Coordinates 4527041..4527409 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUN20_RS21020 (PUN20_21005) 4521324..4523867 + 2544 WP_003140632.1 acyl-homoserine-lactone acylase QuiP -
PUN20_RS21025 (PUN20_21010) 4524022..4525383 - 1362 WP_003081943.1 DNA recombination protein RmuC -
PUN20_RS21030 (PUN20_21015) 4526152..4526907 - 756 WP_003112528.1 RES family NAD+ phosphorylase Toxin
PUN20_RS21035 (PUN20_21020) 4527041..4527409 - 369 WP_003086329.1 DUF2384 domain-containing protein Antitoxin
PUN20_RS21040 (PUN20_21025) 4527482..4528768 - 1287 WP_003086327.1 FAD-binding oxidoreductase -
PUN20_RS21045 (PUN20_21030) 4528824..4530317 - 1494 WP_023130144.1 aldehyde dehydrogenase family protein -
PUN20_RS21050 (PUN20_21035) 4530492..4530968 + 477 WP_003112531.1 hypothetical protein -
PUN20_RS21055 (PUN20_21040) 4530971..4531246 - 276 WP_010792073.1 zinc ribbon domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(63-207)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-47)


Sequences

Toxin        


Download         Length: 252 a.a.        Molecular weight: 28278.42 Da        Isoelectric Point: 7.7294

>T273354 WP_003112528.1 NZ_CP118638:c4526907-4526152 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Download         Length: 756 bp

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13367.63 Da        Isoelectric Point: 9.9874

>AT273354 WP_003086329.1 NZ_CP118638:c4527409-4527041 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Download         Length: 369 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I4U4

Antitoxin

Source ID Structure
AlphaFold DB V6AJU7

References