Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 479205..479823 Replicon chromosome
Accession NC_017634
Organism Escherichia coli O83:H1 str. NRG 857C

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag NRG857_RS02210 Protein ID WP_001291435.1
Coordinates 479205..479423 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag NRG857_RS02215 Protein ID WP_000344800.1
Coordinates 479449..479823 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRG857_RS02175 474492..475064 + 573 WP_000779830.1 YbaY family lipoprotein -
NRG857_RS02180 475095..475406 - 312 WP_000409908.1 MGMT family protein -
NRG857_RS02190 475785..476138 + 354 WP_000878140.1 DUF1428 domain-containing protein -
NRG857_RS02195 476180..477730 - 1551 WP_014640107.1 cyclic-guanylate-specific phosphodiesterase PdeB -
NRG857_RS02200 477894..478364 - 471 WP_000136192.1 YlaC family protein -
NRG857_RS02205 478480..479031 - 552 WP_000102539.1 maltose O-acetyltransferase -
NRG857_RS02210 479205..479423 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
NRG857_RS02215 479449..479823 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
NRG857_RS02220 480367..483516 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
NRG857_RS02225 483539..484732 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T27333 WP_001291435.1 NC_017634:c479423-479205 [Escherichia coli O83:H1 str. NRG 857C]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T27333 NC_017634:c479423-479205 [Escherichia coli O83:H1 str. NRG 857C]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT27333 WP_000344800.1 NC_017634:c479823-479449 [Escherichia coli O83:H1 str. NRG 857C]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT27333 NC_017634:c479823-479449 [Escherichia coli O83:H1 str. NRG 857C]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References