Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 52333..52883 Replicon chromosome
Accession NC_017634
Organism Escherichia coli O83:H1 str. NRG 857C

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag NRG857_RS00245 Protein ID WP_001160966.1
Coordinates 52569..52883 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag NRG857_RS00240 Protein ID WP_000125566.1
Coordinates 52333..52566 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRG857_RS00215 47406..47693 + 288 WP_000203741.1 ferredoxin-like protein FixX -
NRG857_RS00220 47752..49083 + 1332 WP_001183207.1 MFS transporter -
NRG857_RS00225 49191..49721 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
NRG857_RS00230 49714..51576 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
NRG857_RS00235 51768..52247 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
NRG857_RS00240 52333..52566 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
NRG857_RS00245 52569..52883 + 315 WP_001160966.1 CcdB family protein Toxin
NRG857_RS00250 52880..53728 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
NRG857_RS00255 53735..54112 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
NRG857_RS00260 54115..54936 - 822 WP_001065364.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
NRG857_RS00265 54933..55922 - 990 WP_000241228.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
NRG857_RS00270 55922..57208 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T27331 WP_001160966.1 NC_017634:52569-52883 [Escherichia coli O83:H1 str. NRG 857C]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T27331 NC_017634:52569-52883 [Escherichia coli O83:H1 str. NRG 857C]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT27331 WP_000125566.1 NC_017634:52333-52566 [Escherichia coli O83:H1 str. NRG 857C]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT27331 NC_017634:52333-52566 [Escherichia coli O83:H1 str. NRG 857C]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References