Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 86792..88415 Replicon plasmid pNB4833-1
Accession NZ_CP118602
Organism Escherichia coli strain NB4833

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag OU688_RS23800 Protein ID WP_000088046.1
Coordinates 87090..88415 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag OU688_RS23795 Protein ID WP_024136327.1
Coordinates 86792..87085 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OU688_RS23765 (OU688_23765) 82664..83905 + 1242 WP_001053338.1 VWA domain-containing protein -
OU688_RS23770 (OU688_23770) 83996..84127 + 132 WP_000374059.1 hypothetical protein -
OU688_RS23775 (OU688_23775) 84468..84749 - 282 WP_000077926.1 hypothetical protein -
OU688_RS23780 (OU688_23780) 84799..84990 - 192 WP_000398480.1 hypothetical protein -
OU688_RS23785 (OU688_23785) 85082..85423 - 342 WP_001151575.1 hypothetical protein -
OU688_RS23790 (OU688_23790) 85796..86188 + 393 WP_000341066.1 hypothetical protein -
OU688_RS23795 (OU688_23795) 86792..87085 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
OU688_RS23800 (OU688_23800) 87090..88415 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
OU688_RS23805 (OU688_23805) 88476..88682 + 207 WP_000134171.1 hypothetical protein -
OU688_RS23810 (OU688_23810) 88784..89194 + 411 WP_000985911.1 hypothetical protein -
OU688_RS23815 (OU688_23815) 89207..90022 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
OU688_RS23820 (OU688_23820) 90276..90701 + 426 WP_001043843.1 hypothetical protein -
OU688_RS23825 (OU688_23825) 91155..91391 + 237 Protein_89 type I restriction-modification system subunit M N-terminal domain-containing protein -
OU688_RS23830 (OU688_23830) 91446..91745 + 300 WP_001572440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / floR / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / dfrA12 / oqxA / oqxB - 1..261710 261710


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T273286 WP_000088046.1 NZ_CP118602:87090-88415 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT273286 WP_024136327.1 NZ_CP118602:86792-87085 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References