Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4422996..4423493 Replicon chromosome
Accession NZ_CP118601
Organism Escherichia coli strain NB4833

Toxin (Protein)


Gene name ghoT Uniprot ID A0A5F1G8P7
Locus tag OU688_RS21180 Protein ID WP_064525730.1
Coordinates 4422996..4423169 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OU688_RS21185 Protein ID WP_000398619.1
Coordinates 4423197..4423493 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OU688_RS21170 (4419666) 4419666..4421123 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
OU688_RS21175 (4421360) 4421360..4422877 + 1518 WP_001295074.1 lysine--tRNA ligase -
OU688_RS21180 (4422996) 4422996..4423169 - 174 WP_064525730.1 type V toxin-antitoxin system toxin GhoT Toxin
OU688_RS21185 (4423197) 4423197..4423493 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OU688_RS21190 (4423720) 4423720..4423992 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
OU688_RS21195 (4424004) 4424004..4424234 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
OU688_RS21200 (4424415) 4424415..4426046 + 1632 WP_001216460.1 sensor histidine kinase -
OU688_RS21205 (4426043) 4426043..4426762 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6585.09 Da        Isoelectric Point: 10.2288

>T273284 WP_064525730.1 NZ_CP118601:c4423169-4422996 [Escherichia coli]
MTLFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT273284 WP_000398619.1 NZ_CP118601:c4423493-4423197 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5F1G8P7

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References