Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4817260..4817757 Replicon chromosome
Accession NC_017633
Organism Escherichia coli ETEC H10407

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ETEC_RS24165 Protein ID WP_001173343.1
Coordinates 4817584..4817757 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ETEC_RS24160 Protein ID WP_000398619.1
Coordinates 4817260..4817556 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ETEC_RS24135 4813991..4814710 - 720 WP_000611288.1 two-component system response regulator DcuR -
ETEC_RS24140 4814707..4816338 - 1632 WP_001216477.1 sensor histidine kinase -
ETEC_RS24145 4816519..4816749 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
ETEC_RS24150 4816761..4817033 + 273 WP_000405651.1 N-acetyltransferase -
ETEC_RS24160 4817260..4817556 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ETEC_RS24165 4817584..4817757 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ETEC_RS24170 4817876..4819393 - 1518 WP_001295074.1 lysine--tRNA ligase -
ETEC_RS24175 4819630..4821087 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T27326 WP_001173343.1 NC_017633:4817584-4817757 [Escherichia coli ETEC H10407]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T27326 NC_017633:4817584-4817757 [Escherichia coli ETEC H10407]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT27326 WP_000398619.1 NC_017633:4817260-4817556 [Escherichia coli ETEC H10407]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT27326 NC_017633:4817260-4817556 [Escherichia coli ETEC H10407]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References