Detailed information of TA system TA027324

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4258280..4259112
• Replicon: chromosome
• Accession: NC_017633
• Organism: Escherichia coli ETEC H10407

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E3PN67
• Locus tag: ETEC_RS21400
• Protein ID: WP_000854775.1
• Coordinates: 4258738..4259112 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A426P7N5
• Locus tag: ETEC_RS21395
• Protein ID: WP_001285645.1
• Coordinates: 4258280..4258648 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETEC_RS21360	4254103..4254783	+	681	WP_001282911.1	WYL domain-containing protein	-
ETEC_RS21365	4254934..4255611	+	678	WP_001097566.1	hypothetical protein	-
ETEC_RS21370	4255617..4255850	+	234	WP_001278284.1	DUF905 family protein	-
ETEC_RS21375	4255940..4256758	+	819	WP_001175173.1	DUF945 domain-containing protein	-
ETEC_RS21380	4256850..4257335	+	486	WP_000213721.1	antirestriction protein	-
ETEC_RS21385	4257351..4257827	+	477	WP_001186201.1	RadC family protein	-
ETEC_RS21390	4257896..4258117	+	222	WP_000692303.1	DUF987 domain-containing protein	-
ETEC_RS31060	4258117..4258269	+	153	WP_000488333.1	hypothetical protein	-
ETEC_RS21395	4258280..4258648	+	369	WP_001285645.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ETEC_RS21400	4258738..4259112	+	375	WP_000854775.1	TA system toxin CbtA family protein	Toxin
ETEC_RS21405	4259109..4259600	+	492	WP_000976825.1	hypothetical protein	-
ETEC_RS21410	4259612..4259809	+	198	WP_000772035.1	DUF957 domain-containing protein	-
ETEC_RS21415	4259894..4260736	+	843	WP_001274548.1	DUF4942 domain-containing protein	-
ETEC_RS21425	4261532..4262716	+	1185	WP_001172876.1	sugar efflux transporter	-
ETEC_RS21430	4262827..4263750	+	924	WP_000535941.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13965.02 Da
• Isoelectric Point: 8.2830

>T27324 WP_000854775.1 NC_017633:4258738-4259112 [Escherichia coli ETEC H10407]
MKTLPDTLVREASRCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T27324 NC_017633:4258738-4259112 [Escherichia coli ETEC H10407]
ATGAAAACATTACCCGATACACTCGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13678.37 Da
• Isoelectric Point: 5.4492

>AT27324 WP_001285645.1 NC_017633:4258280-4258648 [Escherichia coli ETEC H10407]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTNG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT27324 NC_017633:4258280-4258648 [Escherichia coli ETEC H10407]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGATATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAACGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: E3PN67

Antitoxin

• AlphaFold DB: A0A426P7N5