Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 21573..22710 Replicon plasmid pW6-3
Accession NZ_CP118551
Organism Enterococcus faecium strain W6

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag PWA42_RS14730 Protein ID WP_002332783.1
Coordinates 21573..22436 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag PWA42_RS14735 Protein ID WP_002326825.1
Coordinates 22438..22710 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWA42_RS14710 (PWA42_14710) 17792..18289 + 498 WP_000868795.1 trimethoprim-resistant dihydrofolate reductase DfrG -
PWA42_RS14715 (PWA42_14715) 18361..20313 - 1953 WP_000163792.1 hypothetical protein -
PWA42_RS14720 (PWA42_14720) 20320..20616 - 297 WP_002311824.1 hypothetical protein -
PWA42_RS14725 (PWA42_14725) 20816..21133 - 318 WP_002326830.1 hypothetical protein -
PWA42_RS14730 (PWA42_14730) 21573..22436 - 864 WP_002332783.1 zeta toxin family protein Toxin
PWA42_RS14735 (PWA42_14735) 22438..22710 - 273 WP_002326825.1 antitoxin Antitoxin
PWA42_RS14740 (PWA42_14740) 22728..22943 - 216 WP_001835296.1 peptide-binding protein -
PWA42_RS14745 (PWA42_14745) 23035..23823 - 789 WP_274875504.1 ParA family protein -
PWA42_RS14750 (PWA42_14750) 23936..24889 + 954 WP_010721888.1 IS30 family transposase -
PWA42_RS14755 (PWA42_14755) 24909..24998 - 90 Protein_29 chromosome partitioning protein ParA -
PWA42_RS14760 (PWA42_14760) 25101..25361 - 261 Protein_30 topoisomerase DNA-binding C4 zinc finger domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(M) / tet(L) / dfrG - 1..25672 25672
- flank IS/Tn - - 23936..24889 953


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T273219 WP_002332783.1 NZ_CP118551:c22436-21573 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT273219 WP_002326825.1 NZ_CP118551:c22710-22438 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References