Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1246660..1247577 Replicon chromosome
Accession NZ_CP118541
Organism Bacillus velezensis strain HC-5

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag PWA59_RS06575 Protein ID WP_007407256.1
Coordinates 1246831..1247577 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2C3Z8
Locus tag PWA59_RS06570 Protein ID WP_014417527.1
Coordinates 1246660..1246830 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWA59_RS06520 (1241866) 1241866..1242378 + 513 WP_012117362.1 sigma-70 family RNA polymerase sigma factor -
PWA59_RS06525 (1242491) 1242491..1243042 + 552 Protein_1224 terminase -
PWA59_RS06530 (1243153) 1243153..1243515 + 363 WP_014721043.1 hypothetical protein -
PWA59_RS06535 (1243528) 1243528..1243899 + 372 WP_014417525.1 XkdW family protein -
PWA59_RS06540 (1243904) 1243904..1244101 + 198 WP_007610833.1 XkdX family protein -
PWA59_RS06545 (1244158) 1244158..1244919 + 762 WP_014417526.1 hypothetical protein -
PWA59_RS06550 (1244971) 1244971..1245234 + 264 WP_003154815.1 hemolysin XhlA family protein -
PWA59_RS06555 (1245248) 1245248..1245511 + 264 WP_003154813.1 phage holin -
PWA59_RS06560 (1245525) 1245525..1246403 + 879 WP_007407257.1 N-acetylmuramoyl-L-alanine amidase -
PWA59_RS06565 (1246438) 1246438..1246563 - 126 WP_003154809.1 hypothetical protein -
PWA59_RS06570 (1246660) 1246660..1246830 - 171 WP_014417527.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
PWA59_RS06575 (1246831) 1246831..1247577 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
PWA59_RS06580 (1247681) 1247681..1248679 - 999 WP_003154805.1 inorganic phosphate transporter -
PWA59_RS06585 (1248692) 1248692..1249309 - 618 WP_003154804.1 DUF47 domain-containing protein -
PWA59_RS06590 (1249595) 1249595..1250911 - 1317 WP_007610842.1 amino acid permease -
PWA59_RS06595 (1251233) 1251233..1252183 + 951 WP_014417529.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1232807..1255793 22986


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T273191 WP_007407256.1 NZ_CP118541:c1247577-1246831 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.78 Da        Isoelectric Point: 10.9951

>AT273191 WP_014417527.1 NZ_CP118541:c1246830-1246660 [Bacillus velezensis]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2C3Z8

References