Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 468211..468848 Replicon chromosome
Accession NZ_CP118541
Organism Bacillus velezensis strain HC-5

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PWA59_RS02410 Protein ID WP_003156187.1
Coordinates 468498..468848 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag PWA59_RS02405 Protein ID WP_003156188.1
Coordinates 468211..468492 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWA59_RS02385 (464576) 464576..465175 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
PWA59_RS02390 (465268) 465268..465633 + 366 WP_014304402.1 holo-ACP synthase -
PWA59_RS02395 (465798) 465798..466805 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
PWA59_RS02400 (466922) 466922..468091 + 1170 WP_014416966.1 alanine racemase -
PWA59_RS02405 (468211) 468211..468492 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PWA59_RS02410 (468498) 468498..468848 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PWA59_RS02415 (468966) 468966..469787 + 822 WP_003156182.1 STAS domain-containing protein -
PWA59_RS02420 (469792) 469792..470157 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
PWA59_RS02425 (470160) 470160..470561 + 402 WP_003156178.1 anti-sigma regulatory factor -
PWA59_RS02430 (470573) 470573..471580 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
PWA59_RS02435 (471644) 471644..471973 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
PWA59_RS02440 (471970) 471970..472452 + 483 WP_007609591.1 anti-sigma B factor RsbW -
PWA59_RS02445 (472418) 472418..473206 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
PWA59_RS02450 (473206) 473206..473808 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T273190 WP_003156187.1 NZ_CP118541:468498-468848 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT273190 WP_003156188.1 NZ_CP118541:468211-468492 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References