Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 471913..472550 Replicon chromosome
Accession NZ_CP118495
Organism Bacillus velezensis strain FC02

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PVT72_RS02435 Protein ID WP_003156187.1
Coordinates 472200..472550 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag PVT72_RS02430 Protein ID WP_003156188.1
Coordinates 471913..472194 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PVT72_RS02410 (PVT72_02410) 468278..468877 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
PVT72_RS02415 (PVT72_02415) 468970..469335 + 366 WP_003156192.1 holo-ACP synthase -
PVT72_RS02420 (PVT72_02420) 469500..470507 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
PVT72_RS02425 (PVT72_02425) 470624..471793 + 1170 WP_003156189.1 alanine racemase -
PVT72_RS02430 (PVT72_02430) 471913..472194 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PVT72_RS02435 (PVT72_02435) 472200..472550 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PVT72_RS02440 (PVT72_02440) 472668..473489 + 822 WP_003156182.1 STAS domain-containing protein -
PVT72_RS02445 (PVT72_02445) 473494..473859 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
PVT72_RS02450 (PVT72_02450) 473862..474263 + 402 WP_003156178.1 anti-sigma regulatory factor -
PVT72_RS02455 (PVT72_02455) 474275..475282 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
PVT72_RS02460 (PVT72_02460) 475346..475675 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
PVT72_RS02465 (PVT72_02465) 475672..476154 + 483 WP_003156173.1 anti-sigma B factor RsbW -
PVT72_RS02470 (PVT72_02470) 476120..476908 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
PVT72_RS02475 (PVT72_02475) 476908..477510 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T273133 WP_003156187.1 NZ_CP118495:472200-472550 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT273133 WP_003156188.1 NZ_CP118495:471913-472194 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References