Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5233249..5233874 Replicon chromosome
Accession NZ_CP118405
Organism Klebsiella pneumoniae strain F3-2P(2*)

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HX883_RS25390 Protein ID WP_002882817.1
Coordinates 5233249..5233632 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HX883_RS25395 Protein ID WP_004150355.1
Coordinates 5233632..5233874 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HX883_RS25375 (5230615) 5230615..5231517 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HX883_RS25380 (5231514) 5231514..5232149 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HX883_RS25385 (5232146) 5232146..5233075 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HX883_RS25390 (5233249) 5233249..5233632 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HX883_RS25395 (5233632) 5233632..5233874 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HX883_RS25400 (5234079) 5234079..5234996 + 918 WP_032418237.1 alpha/beta hydrolase -
HX883_RS25405 (5235010) 5235010..5235951 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HX883_RS25410 (5235996) 5235996..5236433 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HX883_RS25415 (5236430) 5236430..5237290 - 861 WP_004146232.1 virulence factor BrkB family protein -
HX883_RS25420 (5237284) 5237284..5237883 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T273123 WP_002882817.1 NZ_CP118405:c5233632-5233249 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT273123 WP_004150355.1 NZ_CP118405:c5233874-5233632 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References