Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 50414..51150 Replicon plasmid unnamed1
Accession NZ_CP118399
Organism Klebsiella pneumoniae strain 2020CK-00223

Toxin (Protein)


Gene name tacT Uniprot ID -
Locus tag PWK27_RS26585 Protein ID WP_064179701.1
Coordinates 50668..51150 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag PWK27_RS26580 Protein ID WP_003026799.1
Coordinates 50414..50680 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWK27_RS26540 (PWK27_26540) 45777..47315 + 1539 WP_004196907.1 IS66-like element ISEc22 family transposase -
PWK27_RS26545 (PWK27_26545) 47605..47868 + 264 WP_009310051.1 hypothetical protein -
PWK27_RS26550 (PWK27_26550) 47865..48431 + 567 WP_009310052.1 hypothetical protein -
PWK27_RS26555 (PWK27_26555) 48462..48956 + 495 WP_009310053.1 hypothetical protein -
PWK27_RS26560 (PWK27_26560) 49000..49368 + 369 WP_009310054.1 hypothetical protein -
PWK27_RS26565 (PWK27_26565) 49399..49602 + 204 WP_004098931.1 HHA domain-containing protein -
PWK27_RS26570 (PWK27_26570) 49651..49908 + 258 WP_004098928.1 hypothetical protein -
PWK27_RS26575 (PWK27_26575) 49984..50238 + 255 WP_004118702.1 hypothetical protein -
PWK27_RS26580 (PWK27_26580) 50414..50680 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
PWK27_RS26585 (PWK27_26585) 50668..51150 + 483 WP_064179701.1 GNAT family N-acetyltransferase Toxin
PWK27_RS26590 (PWK27_26590) 51309..52841 - 1533 WP_009310015.1 IS3-like element ISKpn38 family transposase -
PWK27_RS26595 (PWK27_26595) 52876..53454 - 579 Protein_62 urea ABC transporter permease subunit UrtB -
PWK27_RS26600 (PWK27_26600) 53511..54596 - 1086 Protein_63 urea ABC transporter substrate-binding protein -
PWK27_RS26605 (PWK27_26605) 54684..55710 + 1027 Protein_64 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..136334 136334
- inside IScluster/Tn - - 44980..67154 22174


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17307.95 Da        Isoelectric Point: 9.5822

>T273109 WP_064179701.1 NZ_CP118399:50668-51150 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT273109 WP_003026799.1 NZ_CP118399:50414-50680 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References