Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5223998..5224623 Replicon chromosome
Accession NZ_CP118398
Organism Klebsiella pneumoniae strain 2020CK-00223

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag PWK27_RS25800 Protein ID WP_002882817.1
Coordinates 5223998..5224381 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag PWK27_RS25805 Protein ID WP_004150355.1
Coordinates 5224381..5224623 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWK27_RS25785 (PWK27_25785) 5221364..5222266 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
PWK27_RS25790 (PWK27_25790) 5222263..5222898 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
PWK27_RS25795 (PWK27_25795) 5222895..5223824 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
PWK27_RS25800 (PWK27_25800) 5223998..5224381 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
PWK27_RS25805 (PWK27_25805) 5224381..5224623 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
PWK27_RS25810 (PWK27_25810) 5224828..5225745 + 918 WP_004178029.1 alpha/beta hydrolase -
PWK27_RS25815 (PWK27_25815) 5225759..5226700 - 942 WP_274759448.1 fatty acid biosynthesis protein FabY -
PWK27_RS25820 (PWK27_25820) 5226745..5227182 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
PWK27_RS25825 (PWK27_25825) 5227179..5228039 - 861 WP_004146232.1 virulence factor BrkB family protein -
PWK27_RS25830 (PWK27_25830) 5228033..5228632 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T273105 WP_002882817.1 NZ_CP118398:c5224381-5223998 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT273105 WP_004150355.1 NZ_CP118398:c5224623-5224381 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References