Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4627540..4628350 Replicon chromosome
Accession NZ_CP118398
Organism Klebsiella pneumoniae strain 2020CK-00223

Toxin (Protein)


Gene name KacT Uniprot ID A0A483R2P9
Locus tag PWK27_RS22990 Protein ID WP_048977643.1
Coordinates 4627540..4628073 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag PWK27_RS22995 Protein ID WP_002887278.1
Coordinates 4628084..4628350 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWK27_RS22985 (PWK27_22985) 4626371..4627492 + 1122 WP_002887282.1 cupin domain-containing protein -
PWK27_RS22990 (PWK27_22990) 4627540..4628073 - 534 WP_048977643.1 type II toxin-antitoxin system toxin KacT Toxin
PWK27_RS22995 (PWK27_22995) 4628084..4628350 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
PWK27_RS23000 (PWK27_23000) 4628453..4629886 - 1434 WP_087759796.1 Cu(+)/Ag(+) sensor histidine kinase -
PWK27_RS23005 (PWK27_23005) 4629876..4630559 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
PWK27_RS23010 (PWK27_23010) 4630731..4632116 + 1386 WP_085803494.1 efflux transporter outer membrane subunit -
PWK27_RS23015 (PWK27_23015) 4632134..4632478 + 345 WP_021462623.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(89-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19842.69 Da        Isoelectric Point: 5.2614

>T273102 WP_048977643.1 NZ_CP118398:c4628073-4627540 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGYDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLLSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT273102 WP_002887278.1 NZ_CP118398:c4628350-4628084 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483R2P9

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References