Detailed information of TA system TA273089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3236039..3237759
• Replicon: chromosome
• Accession: NZ_CP118390
• Organism: Edwardsiella piscicida strain Ep21-8

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: D0ZER0
• Locus tag: PWJ79_RS14720
• Protein ID: WP_012849887.1
• Coordinates: 3236039..3236695 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: PWJ79_RS14725
• Protein ID: WP_012849888.1
• Coordinates: 3236692..3237759 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PWJ79_RS14700 (PWJ79_14700)	3231403..3231642	+	240	WP_012849883.1	hypothetical protein	-
PWJ79_RS14705 (PWJ79_14705)	3231681..3234119	+	2439	WP_012849884.1	type I restriction-modification system subunit M	-
PWJ79_RS14710 (PWJ79_14710)	3234113..3235456	+	1344	WP_226989537.1	restriction endonuclease subunit S	-
PWJ79_RS14715 (PWJ79_14715)	3235461..3236036	+	576	WP_012849886.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
PWJ79_RS14720 (PWJ79_14720)	3236039..3236695	+	657	WP_012849887.1	DUF4433 domain-containing protein	Toxin
PWJ79_RS14725 (PWJ79_14725)	3236692..3237759	+	1068	WP_012849888.1	macro domain-containing protein	Antitoxin
PWJ79_RS14730 (PWJ79_14730)	3237923..3241018	+	3096	WP_012849889.1	HsdR family type I site-specific deoxyribonuclease	-
PWJ79_RS14735 (PWJ79_14735)	3241018..3241734	+	717	WP_012849890.1	SprT family zinc-dependent metalloprotease	-
PWJ79_RS14740 (PWJ79_14740)	3241979..3242518	+	540	WP_012849891.1	transposase DNA-binding-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3203579..3259228	55649
	-	flank	IS/Tn	-	-	3242532..3243413	881

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25146.59 Da
• Isoelectric Point: 6.7624

>T273089 WP_012849887.1 NZ_CP118390:3236039-3236695 [Edwardsiella piscicida]
VAYDYSANLNPEKALIWRIVHRDNIPWILDHGLHCGNSPVQAANWINIGNPELIGKRAGHPVPVGMGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSNLRNVAAQNIPFVFTDSHAYYNWVNYYTRLDDLNQIDWSILQARDFRRDPDD
PAKFERYQAEALIWQHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40713.02 Da
• Isoelectric Point: 6.9067

>AT273089 WP_012849888.1 NZ_CP118390:3236692-3237759 [Edwardsiella piscicida]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYAHACKQKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMAWIEDGLQDLRRFLIEKKVRSIAIPPLGAGNGGLNWPDVRARIESTLGDLQDTEILIYEPTEKYQNVAKSS
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEG
LHQWPAGEPWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

Structures

Toxin

• AlphaFold DB: D0ZER0