Detailed information of TA system TA273080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4833481..4834097
• Replicon: chromosome
• Accession: NZ_CP118383
• Organism: Enterobacter hormaechei strain 2020CK-00224

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PWH45_RS23200
• Protein ID: WP_017382676.1
• Coordinates: 4833481..4833852 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: PWH45_RS23205
• Protein ID: WP_015569912.1
• Coordinates: 4833855..4834097 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PWH45_RS23185 (PWH45_23185)	4830981..4831883	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
PWH45_RS23190 (PWH45_23190)	4831880..4832515	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
PWH45_RS23195 (PWH45_23195)	4832512..4833441	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
PWH45_RS23200 (PWH45_23200)	4833481..4833852	-	372	WP_017382676.1	type II toxin-antitoxin system VapC family toxin	Toxin
PWH45_RS23205 (PWH45_23205)	4833855..4834097	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
PWH45_RS23210 (PWH45_23210)	4834296..4835288	+	993	WP_080347228.1	alpha/beta hydrolase	-
PWH45_RS23215 (PWH45_23215)	4835225..4836166	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
PWH45_RS23220 (PWH45_23220)	4836211..4836648	-	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
PWH45_RS23225 (PWH45_23225)	4836645..4837526	-	882	WP_003861949.1	virulence factor BrkB family protein	-
PWH45_RS23230 (PWH45_23230)	4837520..4838119	-	600	WP_017694048.1	glucose-1-phosphatase	-
PWH45_RS23235 (PWH45_23235)	4838238..4839038	-	801	WP_047746063.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13737.88 Da
• Isoelectric Point: 6.4882

>T273080 WP_017382676.1 NZ_CP118383:c4833852-4833481 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T273080 NZ_CP118383:c4833852-4833481 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT273080 WP_015569912.1 NZ_CP118383:c4834097-4833855 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT273080 NZ_CP118383:c4834097-4833855 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATTGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3