Detailed information of TA system TA027306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1623492..1624130
• Replicon: chromosome
• Accession: NC_017633
• Organism: Escherichia coli ETEC H10407

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ETEC_RS08240
• Protein ID: WP_000813794.1
• Coordinates: 1623492..1623668 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ETEC_RS08245
• Protein ID: WP_001270286.1
• Coordinates: 1623714..1624130 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETEC_RS08220	1619111..1620325	-	1215	WP_071597387.1	BenE family transporter YdcO	-
ETEC_RS08225	1620378..1620914	+	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
ETEC_RS08230	1620987..1622948	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ETEC_RS08235	1623040..1623270	-	231	WP_000494244.1	YncJ family protein	-
ETEC_RS08240	1623492..1623668	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ETEC_RS08245	1623714..1624130	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ETEC_RS08250	1624209..1625615	+	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
ETEC_RS08255	1625860..1627005	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
ETEC_RS08260	1627023..1628036	+	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
ETEC_RS08265	1628037..1628978	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1617806..1619071	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T27306 WP_000813794.1 NC_017633:1623492-1623668 [Escherichia coli ETEC H10407]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T27306 NC_017633:1623492-1623668 [Escherichia coli ETEC H10407]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT27306 WP_001270286.1 NC_017633:1623714-1624130 [Escherichia coli ETEC H10407]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT27306 NC_017633:1623714-1624130 [Escherichia coli ETEC H10407]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB