Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4813779..4814137 Replicon chromosome
Accession NC_017632
Organism Escherichia coli UM146

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag UM146_RS29240 Protein ID WP_001332330.1
Coordinates 4814015..4814137 (+) Length 41 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag UM146_RS23880 Protein ID WP_000125566.1
Coordinates 4813779..4814012 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UM146_RS23855 4808852..4809139 + 288 WP_000203741.1 ferredoxin-like protein FixX -
UM146_RS23860 4809198..4810529 + 1332 WP_001183207.1 MFS transporter -
UM146_RS23865 4810637..4811167 + 531 WP_000600749.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
UM146_RS23870 4811160..4813022 + 1863 WP_000377122.1 glutathione-regulated potassium-efflux system protein KefC -
UM146_RS23875 4813214..4813693 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
UM146_RS23880 4813779..4814012 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
UM146_RS29240 4814015..4814137 + 123 WP_001332330.1 CcdB family protein Toxin
UM146_RS29245 4814178..4814330 + 153 WP_146692624.1 CcdB family protein -
UM146_RS23890 4814327..4815175 - 849 WP_000257201.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
UM146_RS23895 4815182..4815559 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
UM146_RS23900 4815562..4816383 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
UM146_RS23905 4816380..4817369 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
UM146_RS23910 4817369..4818655 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-39)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 41 a.a.        Molecular weight: 4563.34 Da        Isoelectric Point: 10.9266

>T27303 WP_001332330.1 NC_017632:4814015-4814137 [Escherichia coli UM146]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Download         Length: 123 bp

>T27303 NC_017632:4814015-4814137 [Escherichia coli UM146]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTGTTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT27303 WP_000125566.1 NC_017632:4813779-4814012 [Escherichia coli UM146]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT27303 NC_017632:4813779-4814012 [Escherichia coli UM146]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References