Detailed information of TA system TA027300

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4636150..4636985
• Replicon: chromosome
• Accession: NC_017632
• Organism: Escherichia coli UM146

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1R2L4
• Locus tag: UM146_RS22990
• Protein ID: WP_001094426.1
• Coordinates: 4636608..4636985 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: UM146_RS22985
• Protein ID: WP_001285575.1
• Coordinates: 4636150..4636518 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UM146_RS22950	4632043..4632723	+	681	WP_001282919.1	WYL domain-containing protein	-
UM146_RS22955	4632871..4633548	+	678	WP_001097301.1	hypothetical protein	-
UM146_RS22960	4633554..4633787	+	234	WP_001278290.1	DUF905 family protein	-
UM146_RS22965	4633877..4634695	+	819	WP_001175160.1	DUF945 domain-containing protein	-
UM146_RS22970	4634786..4635271	+	486	WP_000213697.1	antirestriction protein	-
UM146_RS22975	4635286..4635762	+	477	WP_001298074.1	RadC family protein	-
UM146_RS22980	4635849..4636070	+	222	WP_000692350.1	DUF987 domain-containing protein	-
UM146_RS22985	4636150..4636518	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
UM146_RS22990	4636608..4636985	+	378	WP_001094426.1	TA system toxin CbtA family protein	Toxin
UM146_RS22995	4636982..4637470	+	489	WP_000761683.1	hypothetical protein	-
UM146_RS23000	4637482..4637679	+	198	WP_000839272.1	DUF957 domain-containing protein	-
UM146_RS23005	4639141..4640903	+	1763	Protein_4339	hypothetical protein	-
UM146_RS23010	4641302..4641481	-	180	WP_001298063.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	4623447..4651038	27591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 7.3523

>T27300 WP_001094426.1 NC_017632:4636608-4636985 [Escherichia coli UM146]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T27300 NC_017632:4636608-4636985 [Escherichia coli UM146]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT27300 WP_001285575.1 NC_017632:4636150-4636518 [Escherichia coli UM146]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT27300 NC_017632:4636150-4636518 [Escherichia coli UM146]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454ABD6

Antitoxin

• AlphaFold DB: A0A454ABF5