Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4365968..4366465 Replicon chromosome
Accession NC_017632
Organism Escherichia coli UM146

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag UM146_RS21655 Protein ID WP_001173343.1
Coordinates 4366292..4366465 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag UM146_RS21650 Protein ID WP_001312331.1
Coordinates 4365968..4366264 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UM146_RS21630 4362698..4363417 - 720 WP_000611291.1 two-component system response regulator DcuR -
UM146_RS21635 4363414..4365045 - 1632 WP_001216482.1 sensor histidine kinase -
UM146_RS21640 4365226..4365456 + 231 WP_000373293.1 (4Fe-4S)-binding protein -
UM146_RS21645 4365468..4365740 + 273 WP_000405647.1 N-acetyltransferase -
UM146_RS21650 4365968..4366264 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
UM146_RS21655 4366292..4366465 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
UM146_RS21660 4366584..4368101 - 1518 WP_000003806.1 lysine--tRNA ligase -
UM146_RS21665 4368338..4369795 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T27299 WP_001173343.1 NC_017632:4366292-4366465 [Escherichia coli UM146]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T27299 NC_017632:4366292-4366465 [Escherichia coli UM146]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT27299 WP_001312331.1 NC_017632:4365968-4366264 [Escherichia coli UM146]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT27299 NC_017632:4365968-4366264 [Escherichia coli UM146]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References