Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3120228..3120846 Replicon chromosome
Accession NC_017632
Organism Escherichia coli UM146

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag UM146_RS15640 Protein ID WP_001291435.1
Coordinates 3120628..3120846 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag UM146_RS15635 Protein ID WP_000344800.1
Coordinates 3120228..3120602 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UM146_RS15625 3115318..3116511 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
UM146_RS15630 3116534..3119683 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
UM146_RS15635 3120228..3120602 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
UM146_RS15640 3120628..3120846 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
UM146_RS15645 3121020..3121571 + 552 WP_000102543.1 maltose O-acetyltransferase -
UM146_RS15650 3121687..3122157 + 471 WP_000136192.1 YlaC family protein -
UM146_RS15655 3122321..3123871 + 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
UM146_RS15660 3123913..3124266 - 354 WP_000878140.1 DUF1428 domain-containing protein -
UM146_RS15665 3124645..3124956 + 312 WP_000409908.1 MGMT family protein -
UM146_RS15670 3124987..3125559 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T27296 WP_001291435.1 NC_017632:3120628-3120846 [Escherichia coli UM146]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T27296 NC_017632:3120628-3120846 [Escherichia coli UM146]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT27296 WP_000344800.1 NC_017632:3120228-3120602 [Escherichia coli UM146]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT27296 NC_017632:3120228-3120602 [Escherichia coli UM146]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References