Detailed information of TA system TA027294

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2501596..2502394
• Replicon: chromosome
• Accession: NC_017632
• Organism: Escherichia coli UM146

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1RDD2
• Locus tag: UM146_RS12645
• Protein ID: WP_001094434.1
• Coordinates: 2501596..2501970 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: UM146_RS12650
• Protein ID: WP_001552747.1
• Coordinates: 2502017..2502394 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UM146_RS12610	2497185..2497739	-	555	WP_001001909.1	molecular chaperone YcdY	-
UM146_RS12615	2497763..2498500	-	738	WP_000283664.1	zinc-binding phosphatase	-
UM146_RS12620	2498555..2499493	-	939	WP_000351298.1	glyoxylate/hydroxypyruvate reductase GhrA	-
UM146_RS12630	2499964..2500806	-	843	WP_001280460.1	DUF4942 domain-containing protein	-
UM146_RS12635	2500899..2501096	-	198	WP_000839257.1	DUF957 domain-containing protein	-
UM146_RS12640	2501108..2501599	-	492	WP_000976859.1	hypothetical protein	-
UM146_RS12645	2501596..2501970	-	375	WP_001094434.1	TA system toxin CbtA family protein	Toxin
UM146_RS12650	2502017..2502394	-	378	WP_001552747.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
UM146_RS12655	2502444..2503088	-	645	WP_000086759.1	hypothetical protein	-
UM146_RS12660	2503107..2503328	-	222	WP_000692315.1	DUF987 domain-containing protein	-
UM146_RS12665	2503391..2503867	-	477	WP_001186727.1	RadC family protein	-
UM146_RS12670	2503883..2504356	-	474	WP_001542276.1	antirestriction protein	-
UM146_RS12675	2504450..2504695	-	246	WP_001164966.1	hypothetical protein	-
UM146_RS12680	2504695..2505516	-	822	WP_001234585.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13921.82 Da
• Isoelectric Point: 6.8517

>T27294 WP_001094434.1 NC_017632:c2501970-2501596 [Escherichia coli UM146]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T27294 NC_017632:c2501970-2501596 [Escherichia coli UM146]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13757.63 Da
• Isoelectric Point: 6.3127

>AT27294 WP_001552747.1 NC_017632:c2502394-2502017 [Escherichia coli UM146]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRTGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT27294 NC_017632:c2502394-2502017 [Escherichia coli UM146]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCACCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCTGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCACCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: Q1RDD2