Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1324018..1324934 Replicon chromosome
Accession NZ_CP118167
Organism Bacillus subtilis strain 21855

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag PUW21_RS06950 Protein ID WP_003244695.1
Coordinates 1324188..1324934 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag PUW21_RS06945 Protein ID WP_003232646.1
Coordinates 1324018..1324188 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUW21_RS06910 (1320881) 1320881..1321210 + 330 WP_003232660.1 XkdW family protein -
PUW21_RS06915 (1321207) 1321207..1321371 + 165 WP_003232658.1 XkdX family protein -
PUW21_RS06920 (1321415) 1321415..1322254 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
PUW21_RS06925 (1322307) 1322307..1322576 + 270 WP_003232655.1 hemolysin XhlA family protein -
PUW21_RS06930 (1322589) 1322589..1322852 + 264 WP_003232653.1 phage holin -
PUW21_RS06935 (1322865) 1322865..1323758 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
PUW21_RS06940 (1323795) 1323795..1323932 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
PUW21_RS06945 (1324018) 1324018..1324188 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
PUW21_RS06950 (1324188) 1324188..1324934 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
PUW21_RS06955 (1325044) 1325044..1326045 - 1002 WP_003232642.1 inorganic phosphate transporter -
PUW21_RS06960 (1326058) 1326058..1326675 - 618 WP_003218470.1 DUF47 domain-containing protein -
PUW21_RS06965 (1326951) 1326951..1328267 - 1317 WP_003244819.1 serine/threonine exchanger -
PUW21_RS06970 (1328656) 1328656..1329606 + 951 WP_003245086.1 ring-cleaving dioxygenase -
PUW21_RS06975 (1329707) 1329707..1329853 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1288435..1329853 41418


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T272926 WP_003244695.1 NZ_CP118167:c1324934-1324188 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT272926 WP_003232646.1 NZ_CP118167:c1324188-1324018 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References