Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518264..518900 Replicon chromosome
Accession NZ_CP118167
Organism Bacillus subtilis strain 21855

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PUW21_RS02640 Protein ID WP_003156187.1
Coordinates 518550..518900 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag PUW21_RS02635 Protein ID WP_003225183.1
Coordinates 518264..518545 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUW21_RS02615 (514623) 514623..515222 - 600 WP_032722928.1 rhomboid family intramembrane serine protease -
PUW21_RS02620 (515317) 515317..515682 + 366 WP_015252768.1 holo-ACP synthase -
PUW21_RS02625 (515848) 515848..516864 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
PUW21_RS02630 (516979) 516979..518148 + 1170 WP_015252766.1 alanine racemase -
PUW21_RS02635 (518264) 518264..518545 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PUW21_RS02640 (518550) 518550..518900 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PUW21_RS02645 (519015) 519015..519839 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
PUW21_RS02650 (519844) 519844..520209 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
PUW21_RS02655 (520213) 520213..520614 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
PUW21_RS02660 (520626) 520626..521633 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
PUW21_RS02665 (521695) 521695..522024 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
PUW21_RS02670 (522021) 522021..522503 + 483 WP_003234299.1 anti-sigma B factor RsbW -
PUW21_RS02675 (522469) 522469..523257 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
PUW21_RS02680 (523257) 523257..523856 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T272925 WP_003156187.1 NZ_CP118167:518550-518900 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT272925 WP_003225183.1 NZ_CP118167:518264-518545 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References