Detailed information of TA system TA272847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 118275..119983
• Replicon: chromosome
• Accession: NZ_CP118147
• Organism: Pseudomonas chlororaphis strain ATCC 174151

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: PUP56_RS00580
• Protein ID: WP_114761240.1
• Coordinates: 118275..119153 (+)
• Length: 293 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A5M7C4U8
• Locus tag: PUP56_RS00585
• Protein ID: WP_007928502.1
• Coordinates: 119150..119983 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PUP56_RS00555 (PUP56_00555)	114283..115119	+	837	WP_223816097.1	ABC transporter ATP-binding protein	-
PUP56_RS00560 (PUP56_00560)	115159..116148	+	990	WP_114761241.1	ABC transporter substrate-binding protein	-
PUP56_RS00565 (PUP56_00565)	116177..116953	+	777	WP_007928506.1	ABC transporter permease	-
PUP56_RS00570 (PUP56_00570)	116953..117717	+	765	WP_101336297.1	SDR family oxidoreductase	-
PUP56_RS00575 (PUP56_00575)	117731..118270	+	540	WP_085532783.1	cupin domain-containing protein	-
PUP56_RS00580 (PUP56_00580)	118275..119153	+	879	WP_114761240.1	alpha/beta hydrolase	Toxin
PUP56_RS00585 (PUP56_00585)	119150..119983	+	834	WP_007928502.1	IclR family transcriptional regulator	Antitoxin
PUP56_RS00590 (PUP56_00590)	119985..120782	+	798	WP_114761239.1	SDR family oxidoreductase	-
PUP56_RS00595 (PUP56_00595)	120775..122457	+	1683	WP_114761268.1	thiamine pyrophosphate-binding protein	-
PUP56_RS00600 (PUP56_00600)	122468..123271	+	804	WP_085532775.1	aspartate dehydrogenase	-
PUP56_RS00605 (PUP56_00605)	123283..124779	+	1497	WP_114761238.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (45-276)

Antitoxin

• Transcription regulator IclR, C-terminal (136-256)

Sequences

Toxin

Protein

• Length: 293 a.a.
• Molecular weight: 31023.53 Da
• Isoelectric Point: 8.0027

>T272847 WP_114761240.1 NZ_CP118147:118275-119153 [Pseudomonas chlororaphis]
MLPGLSQPVSTADLQARLTRDFAQRQVTVAGGRQALRECGAGPAIVLLHGIGSGAASWLQVAQQLAPQARVIAWDAPGYG
DSSPLESPAPKAEHYAARLVQMLDALDVEECVLVGHSLGALPALAFARSSQASRVSRLVLISPARGYGAPERGEQRQQVR
RQRLENLEHLGITGLAEQRSTHMLSPNAAPQALAWVRWNMARLNPAGYRQAIELLCGGDLLRHGQPGMPCEVHCGEADRI
TPAETCLTLAKALGASFNLIADAGHASPIEQPLVVAGRLAFAIKESLTGRTL

Nucleotide

• Length: 879 bp

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30869.31 Da
• Isoelectric Point: 6.6493

>AT272847 WP_007928502.1 NZ_CP118147:119150-119983 [Pseudomonas chlororaphis]
MNDSDPLKDAQDKYIVPGLERGLLLLCEFSRRNRTLTAPELARRLALPRSTIFRLLTTLETMGFVTRSGNEYRLGMSVLR
LGFEYLASLELTELGQPLLARLCDRLNYPSNLVVRDGRSIVYVAKVSPPSVFSNAVNVGTRLPAHATVLGRILLEDLSLA
ELRELYPEEHLEQYSHSTPKTVLELFDMVQADRQRGFISGEGFFESAISTVAAPVRDQSGRIAAALGVTIPTTQIGHVNF
EELLSQVRRSADELSRLLNYNGVAHDGQQRVTALMRD

Nucleotide

• Length: 834 bp

Structures

Antitoxin

• AlphaFold DB: A0A5M7C4U8