Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 563402..564015 Replicon chromosome
Accession NZ_CP118146
Organism Pseudomonas chlororaphis strain ATCC 174152

Toxin (Protein)


Gene name hicA Uniprot ID A0A285EYZ4
Locus tag PUP66_RS02525 Protein ID WP_009041685.1
Coordinates 563402..563584 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2N8BII0
Locus tag PUP66_RS02530 Protein ID WP_009041686.1
Coordinates 563614..564015 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUP66_RS02495 (PUP66_02495) 559167..559424 - 258 WP_062824009.1 hypothetical protein -
PUP66_RS02500 (PUP66_02500) 559477..559965 - 489 WP_009041680.1 DoxX family protein -
PUP66_RS02505 (PUP66_02505) 560039..560935 - 897 WP_114760179.1 SDR family oxidoreductase -
PUP66_RS02510 (PUP66_02510) 560965..561474 - 510 WP_009041682.1 DUF1097 domain-containing protein -
PUP66_RS02515 (PUP66_02515) 561508..562248 - 741 WP_053259257.1 SDR family oxidoreductase -
PUP66_RS02520 (PUP66_02520) 562387..563277 + 891 WP_053259258.1 LysR family transcriptional regulator -
PUP66_RS02525 (PUP66_02525) 563402..563584 + 183 WP_009041685.1 type II toxin-antitoxin system HicA family toxin Toxin
PUP66_RS02530 (PUP66_02530) 563614..564015 + 402 WP_009041686.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
PUP66_RS02535 (PUP66_02535) 564104..565150 - 1047 WP_114760180.1 LacI family DNA-binding transcriptional regulator -
PUP66_RS02540 (PUP66_02540) 565358..567616 + 2259 WP_114760181.1 TonB-dependent receptor -
PUP66_RS02545 (PUP66_02545) 567663..568988 + 1326 WP_009041689.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.08 Da        Isoelectric Point: 10.4588

>T272841 WP_009041685.1 NZ_CP118146:563402-563584 [Pseudomonas chlororaphis]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Download         Length: 183 bp

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14629.48 Da        Isoelectric Point: 4.7569

>AT272841 WP_009041686.1 NZ_CP118146:563614-564015 [Pseudomonas chlororaphis]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAEAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGMI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Download         Length: 402 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285EYZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A2N8BII0

References